Choose the correct order for the formation of a recombinant organism and the expresssion of the
desired product.
I’m stuck on this one any advice?

Choose The Correct Order For The Formation Of A Recombinant Organism And The Expresssion Of Thedesired

Answers

Answer 1

The order for rDNA is to identify a target gene, select a plasmid, use a restriction enzyme, gene insertion, use ligase, introduce the recombinant DNA and detect recombinant cells. rDNA involves genetic recombination.

Recombinant DNA (rDNA) technologies are used in molecular biology laboratories to create chimeric DNA molecules containing nucleotide sequences from different species.

Recombinant DNA techniques can be used to identify and sequence genes, as well as to discover gene function.

The sequential steps of rDNA technologies are as follow:

Identify a gene of interest.Select a DNA plasmid to be used.Use restriction enzymes to cleave the plasmid DNA.Introduce the gene of interest in the plasmid.Use ligase enzymes to join both nucleotide sequences.Introduce the recombinant DNA molecule into the host cell.Detect and replicate the cells containing the desired recombinant DNA molecule.

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Related Questions

photosynthesis and cellular respiration scientific principles

Answers

Explanation:

Photosynthesis makes the glucose that is used in cellular respiration to make ATP. ... While photosynthesis requires carbon dioxide and releases oxygen, cellular respiration requires oxygen and releases carbon dioxide. It is the released oxygen that is used by us and most other organisms for cellular respiration.

Question 6
The electrical charge of a particle called

Answers

Answer:

The electrical charge of a particle is called an electric charge.

Explanation:

Electricity always comes in units of the Coulomb, which is the unit of measurement for electric charges. One Coulomb is defined as follows: "the quantity or magnitude of electricity carried by an electron in the electrostatic field around a point on the Earth with coordinates (latitude, longitude)". We can use units like volts and amperes to communicate this kind of data now, but typically you'll see it written as Cs.  

To do some simple arithmetic with these units you need to know that one Couloth will equal 93 millimeters squared per second times per second (mC/s*ms).

What is the function of the cell membrane? The cell membrane controls what goes into the cell (food) and what leaves the cell (waste). The cell membrane protects the cell from damage. The cell membrane controls the amount of water that goes into the cell and stores it there if it doesn't rain. The cell membrane allows air to enter, but won't allow water to enter into the cell.

Answers

Answer:

Cell membranes serve as barriers and gatekeepers. They are semi-permeable, which means that some molecules can diffuse across the lipid bilayer but others cannot. Small hydrophobic molecules and gases like oxygen and carbon dioxide cross membranes rapidly

Explanation:

How does reproduction in humans compare to reproduction and other animals such as fish, birds and reptiles?

Answers

Answer:

It compares because all of them reproduce the same way with varied offspring instead of identical.

Which pair of triangles can be proven congruent by SAS?

Answers

Answer:

Explanation:

The seconded one

The second triangle can be proven congruent by SAS (side-angle-side). Therefore, option B is correct.

What is SAS property?

SAS stands for "Side-Angle-Side" and it is a property of congruent triangles. This property states that if two triangles have two pairs of corresponding sides that are proportional and the included angles between those sides are congruent, then the triangles are congruent.

In other words, if two triangles have the same length of two sides and the angles formed by these sides are the same, then the triangles are congruent.

This property is one of several ways to prove that two triangles are congruent, and it is a useful tool in geometry and trigonometry. The other ways to prove the congruence of triangles are SSS (Side-Side-Side), ASA (Angle-Side-Angle), AAS (Angle-Angle-Side), and HL (Hypotenuse-Leg) properties. Hence, option B is correct.

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Biology which statement applies to cholesterol?
A. It is hydrophobic and found on the outside of the phospholipid biyaler
B. It is hydrophilic and found inside the phospholipid bilayer
C. It impacts membrane fluidity.
D. It is transported in association with glucose in the blood.

Answers

The true statement about cholesterol is that; " It impacts membrane fluidity."

Cholesterol is one of the steroids in the body and serves as a constituent of many hormones. Cholesterol consists of a hydrophilic and a hydrophobic part hence it is able to interact both with aqueous and nonpolar environments.

Cholesterol impacts membrane fluidity in the sense that it regulates the direction of membrane fluidity. At high temperatures, cholesterol raises the membrane temperature whereas at high temperatures, it intercalates between phospholipids thereby preventing stiffening.

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How the animals survive in tropical rainforest

Answers

Answer:

The animals use the tall trees for shelter, hiding places from their predators, and source of food. Because there are many animals competing for food, many animals have adapted by learning to eat a particular food eaten by no there animal

Explanation:

Put in own words and add more reasons and details hope it helped a lil

What is the role of transgenic organisms in the production
of medicine and agriculture?
HELP PLA

Answers

Explanation:

Transgenic animals are routinely bred to carry human genes, or mutations in specific genes, thus allowing the study of the progression and genetic determinants of various diseases.

Transgenesis allows improvement of nutrients in animal products, including their quantity, the quality of the whole food, and specific nutritional composition.

How the form of ostrich’s eggs help the bird survive?

Answers

Answer:

Adaptations that make ostriches fast on land but unable to fly include long legs with strong muscles, thick bones and powerful 2-toed feet

Need questions 2-11 done pls

Answers

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In mitosis, the daughter cells have the same number of chromosomes as the parent cell, while in meiosis, the daughter cells have half the number of chromosomes as the parent.meiosis gives rise to four unique daughter cells, each of which has half the number of chromosomes as the parent cell.A haploid cell is a germ cell or reproductive cell-like egg or sperm that has a single set of chromosomes and it is represented by n. A diploid cell is a bodily cell or somatic cell that has two sets of chromosomes( one set from the paternal line and one set from the maternal line)From a diploid germ cell 2n =24 double stranded chromosomes, meiosis will produce four gamete haploid cells n =12 single stranded chromosomes.ovumspermGenetic variation is increased by meiosis Because of recombination and independent assortment in meiosis, each gamete contains a different set of DNA. filter that blocks out unwanted frequencies to a speaker or group of speakersdescribes how different genes independently separate from one another when reproductive cells develop. I d k the last one sorry

hope it helps

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Why don't people's cells starve from lack of glucose while they are fasting​

Answers

Answer:


Fasting can definitely raise blood glucose. This is due to the effect of insulin falling and the rising counter-regulatory hormones including increased sympathetic tone, noradrenaline, cortisol and growth hormone, in addition to glucagon. These all have the effect of pushing glucose from liver storage into the blood. This is normal. If you are not eating, you want to use some stored glucose. The question is this – if you are not eating, and your blood glucose went up, where did that glucose come from? It can only have come from your own body (liver). So, it’s a natural phenomenon, and the fasting now allows your body to use some of the glucose for energy.


Capturing gas emitted from landfills results in a(n) _____________ of CO2 production.

Question 3 options:

a)

increase


b)

decrease


c)

balance

Answers

Answer:

b. decrease

Explanation:

that's only my answer but by thinking the capturing gas emitted from landfills results in decrease of CO2 production.

Writing your own quiz from the information you learned in class and during your reading will help improve your study skills.
A.
True
B.
False

Answers

A. True
Ur quizzing yourself on what you learned. That is a great way of remembering what you learned.

help now asap plssssss ols dont answer if you dont know​

Answers

Answer:

telescope

Explanation:

Answer:

Optical Telescopes , Radio Telescopes , Spectroscopes , Star Charts

Explanation:

How the food you eat affects your brain?

Answers

Answer:

Eating high-quality foods that contain lots of vitamins, minerals, and antioxidants nourishes the brain and protects it from oxidative stress — the "waste" (free radicals) produced when the body uses oxygen, which can damage cells. ... Diets high in refined sugars, for example, are harmful to the brain.

What is the most likely mode of inheritance? Which individuals from the pedigree exclude other models of inheritance?
PLZ HELP

Answers

Answer:

The modes of inheritance are autosomal dominant , autosomal recessive, and X-linked. To simplify the discussion of these different forms, the trait used in the following text will be a hereditary disease.

Brainliest plz

Is it possible to see the different phases of mitosis in plant cells under a low power microscope? A yes, you can see the individual cells and tell the phases apart. B yes, you can see the individual cells and they are all just in the same phase. C No, you can't see enough detail. They all look like they are in interphase. D. No, there is not enough detail to see individual parts of the plant or individual cells. ​

Answers

It is not possible to see the different phases of mitosis in plant cells under a low-power objective microscope.

Under low-power objectives, individual plant cells can be seen as room-like compartments but the essential details of the contents of the cells cannot be seen. Thus, all the cells appear the same as if they are in an interphase phase of the cell cycle.

In order to view the different phases of mitosis in plant cells, higher magnifications such as high power would be required.

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A local watershed carries runoff from farms, suburban lawns, and wastewater treatment plants into local rivers, and eventually into a local bay. How could this affect the health of the bay?
A. It could make water hypoxic for organisms.
B. There will be an increase of aquatic plants and fish.
C. It could increase dissolved oxygen for fish.
D. There will be decreased nutrients in the water.

Answers

Answer:

answer should be "making water hypoxic for organisms". reason is because the runoff from these treatment plants are filled with nutrients, which will cause eutrophication. eutrophication is excessive algae sprouts which create more DO but then later becomes all used up when they decompose, making it so other organisms have no oxygen.

The runoff water from farms, suburban lawns, and wastewater treatment plants into local rivers affects the health of the bay by making water more hypoxic for organisms. Thus, the correct option is A.

What is Local bay?

A Local bay may be defined as an area of the sea or other water bodies which are usually smaller than a gulf. It is mostly surrounded by water.

The runoff water from farms, suburban lawns, and wastewater treatment plants contain an enormous amount of nutrients that assist the growth of algae over the surface of water bodies known as eutrophication.

These algae consume all much of the available oxygen of the water body and make the environment hypoxic i.e. oxygen deficient.

Therefore, the correct option for this question is A.

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Protecting shoreline communities from tsunamis is a struggle, especially in less developed regions of the world. Explain why your experiment supports this statement.

Answers

Protecting shoreline communities from tsunamis is a struggle, especially in less developed regions of the world because tsunamis are unpredictable. These phenomena have a higher effect on low-income shoreline families.

Tsunamis can be defined as giant ocean waves due to earthquakes and/or volcanic activity that occur under the sea.

Tsunamis may devastate entire shoreline communities because they are unpredictable and they speed along like a jet plane.

Tsunamis mainly affect shoreline low-income families in developing countries because the houses in which these people live in developing countries are rudimentary.

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what is the relationship between DNA Chromosomes and organism?

Answers

Genes are segments of deoxyribonucleic acid (DNA) that contain the code for a specific protein that functions in one or more types of cells in the body. Chromosomes are structures within cells that contain a person's genes. Genes are contained in chromosomes, which are in the cell nucleus.

OMG HELP RN

The following chart below show the conductors and insulators experimented with in the circuits gizmo. The chart has been filled in incorrectly with some insulators and conductors in the wrong column.



True
False

Answers

Answer:

TRUE

Explanation:

it’s true i just finished that worksheet

After following humane guidelines for stunning an
animal, the carcass is

Answers

sorry I don’t know I just need points

three cell organelles found in plant and animal cell

Answers

Answer:

Image result for three cell organelles found in plant and animal cell

Structurally, plant and animal cells are very similar because they are both eukaryotic cells. They both contain membrane-bound organelles such as the nucleus, mitochondria, endoplasmic reticulum, golgi apparatus, lysosomes, and peroxisomes. Both also contain similar membranes, cytosol, and cytoskeletal elements.

What is the food web for Altamaha shiner ?

Answers

Answer:

The bottom of the pyramid was fertilizer or something and the little top triangle was a silhouette of a man. Those were simple days and the food chain seemed simpler.

Explanation:

What occurs if kelp is not present to absorb CO2 or produce O2?

Answers

there won't be fresh oxygen and CO2 will be dangerous for out health and we may not have some of the energy that we have today because kelp also can produce energy.

All other living organisms in the aquatic environment will be negatively affected if Kelp is not present to absorb CO2 or produce O2.

ROLE OF PHOTOSYNTHESIS:

Kelp are living organisms in the kingdom Protista, which possess the ability to photosynthesize or use energy from sunlight to produce food.

Photosynthesis is a process that absorbs CO2 from the atmosphere and release O2 as a gaseous product.

Kelp is a producer of aquatic environment, which means it helps to absorb CO2 and release O2 into the atmosphere. This O2 is used for cellular respiration by other living organisms.

If kelp is not present to absorb CO2 or produce O2, other other living organisms in the aquatic environment will be negatively affected.

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Select all that apply: Which of the following is NOT a location for a synovial joint
A. structures of the skull
B. Ankle joints
C. Finger joints
D. Rib joints

Answers

B, C, and D. I think

Name at least three hormones which are used commercially and list why they are used.

Answers

Answer:

Auxins, Ethene and Cytopkinins

Explanation:

Auxins used in commercial fo Crop production.

Ethene used in commercial to fruit to ripen.

Cytokinins used in commercial to reduce yellowing in green vegetables.

Auxins are utilized in the commercial production of crops. Ethene is a commercial fruit ripening agent. Cytokinins are used commercially to stop green crops from turning yellow.

What are plant hormones?

Almost every element of plant growth and reaction to its environment is regulated by plant hormones. Numerous plant hormones play important roles in the interactions between plants and helpful microorganisms because they are active at extremely low concentrations and have precise spatial control of synthesis and response.

The "The Role of Plant Hormones in Plant-Microbe Symbioses" special issue offers fresh information on the functions of hormones produced from both the plant and the microbial partner in symbiosis establishment and communication. This includes the close symbioses that the majority of terrestrial plants have with arbuscular mycorrhizal (AM) fungi, as well as the more recently acquired nodulation—symbioses between a select group of plants in the fabid clade and nitrogen-fixing bacteria.

The articles in this special issue also look at how hormones affect how plants interact with ectomycorrhizae, endophytic bacteria, and fungi, and helpful microorganisms that stick to the surfaces of the leaves and roots. Many hormones can interact with one another in addition to acting directly to regulate the growth of these symbioses, and these intricate networks are just beginning to emerge.

Therefore, various plant hormones are used commercially.

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Compare the population growth rate in industrially developed countries to that in developing countries

Answers

Answer:

The worlds developing regions will see 1.2 billion people added, a 20.7% increase; while the population of developed countries will increase a mere 3.3% adding 41 million to the current 1.3 billion people. ... There is a difference of more than ten years between the median age in developing and developed regions.

The population growth rate in industrially developed countries is much lower than that in developing countries.

The population growth rate is the number of births per 1,000 people in a population in a year. The population growth rate in industrially developed countries is typically around 1%, while the population growth rate in developing countries is typically around 2%.

There are several reasons why the population growth rate is lower in industrially developed countries. First, people in industrially developed countries tend to have fewer children. This is due to a number of factors, including the availability of birth control, the increasing participation of women in the workforce, and the rising cost of raising children.

Second, people in industrially developed countries tend to live longer. This is due to advances in medicine and healthcare. As a result, the number of people in the population who are of childbearing age is lower in industrially developed countries than in developing countries.

The lower population growth rate in industrially developed countries has a number of implications. First, it means that the population in these countries is aging. This is because the number of people who are of working age is shrinking, while the number of people who are retired is growing.

Second, the lower population growth rate means that there is less pressure on resources such as land, water, and food. This can help to reduce poverty and improve the standard of living in these countries.

However, the lower population growth rate also means that there is a smaller pool of workers to support the economy. This can lead to labor shortages and economic problems.

Overall, the lower population growth rate in industrially developed countries has both positive and negative implications. It is important to carefully consider these implications when making policy decisions.

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Which structure is most directly responsible for maintain homeostasis in all the cells?

Answers

Answer:

Cell membrane or plasma membrane is most directly responsible for maintain homeostatis in all cells.

How can antiviral medications help someone who has the flu?
- helps the person sleep better so that they heal
- prevents the person from ever getting the flu
- makes the person feel better faster and makes the symptoms milder
- cures the person immediately (I know its not this one)

Answers

Answer:

Prevents the person from ever getting the flu. (better said as 'prevents attracting flu in the future")

Explanation:

Antiviral drugs are recommended for both treatment and prevention of flu. They can protect you from getting viral infections or spreading a virus to others.

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