In whole-genome shotgun sequencing, the chromosome is cut into pieces, the pieces are sequenced, and the sequences are aligned based on overlapping sequences into a contig. Place the fragments of DNA in the proper places to form the contig for this sequence of DNA, using the central CAT sequence as a guide. ResetHelp CATCGAGCATCGAG CCAGGCCCCAGGCC GGCCATCGGCCATC AGGCCATAGGCCAT CCATCGACCATCGA

Answers

Answer 1

Answer:

CCAGGCC

CCATCGA

GGCCATC

CAT

AGGCCAT

CATCGAG

Explanation:

Shotgun sequencing is a method used to determine the nucleotide sequence of entire chromosomes/genomes. This sequencing method consists of obtaining random DNA fragments which are subsequently classified by bioinformatic tools that ordering them according to overlapping sequences called contigs. In the whole-genome shotgun (WGS) technique, the entire genome of an organism is sequenced, being the critical factor the depth of sequencing, which refers to the quality of the sequencing reads (e.g., a depth of 20X indicates that the genome is sequenced 20 times by a sequencing machine). For the human genome, WGS became available after the completion of the Human Genome Project (HGP), which enabled the generation of a reference sequence for the whole human genome. The steps of the WGS technique are the following:

1. Preparation of isolated chromosomes

2. The DNA is sheared into small fragments

3. The DNA fragments of about 1 kilobase (1000 base pairs) are incorporated into plasmids which are cloned to render pure samples of each DNA fragment

4. The plasmid clones are sequenced by sequencing machines

5. Bioinformatic tools finally are used to link DNA fragments by their overlapping ends


Related Questions

can you please help me ​

Answers

Answer: 15. Oxygen. 16. Cortex. 17. epiglottis

Explanation:

Based on the information given above, which of the following models best represents the relationship between zooplankton , minnows, and largemouth bass in a freshwater ecosystem?
Chose the correct image to answer the question above. NEED THIS FAST

Answers

Answer:

Option C: Minnows have a direct negative effect (⇒(-)) on the population size of zooplankton. Basses have a negative effect (⇒(-)) on the population size of minnows. Basses have an indirect positive  (---> (+)) effect on zooplankton.

Explanation:

In the trophic web occurs a process of energy transference through different organisms that places at different levels. Each level feeds on the preceding one and becomes food for the next one. The first link is an autotroph organism or producer. The following links are the consumers: herbivores are primary consumers and feed on producers. Carnivores are secondary consumers and feed on herbivores, and so on. The last links are the decomposers, microorganisms that act on dead animals degrading organic matter.

If any of the links change their population size (increasing it or decreasing it), it affects the superior links and the immediately anterior link.

In the exposed example, zooplankton, minnows, and basses are continuously interacting.

Minnow →(-) Zooplankton

Minnows feed on zooplankton. So they affect direct and negatively the invertebrate population. The more minnows there are, the more zooplankton they eat. So, as long as the minnows population keeps growing, the zooplankton population will be more affected because they will not have enough time to reproduce and recover from predation.

Bass →(-) Minnows

Bass feed on minnows, so they also affect direct and negatively the fish population. And the more the bass population grows in size, the more affected the minnows will be because many more basses will be eating them.

Bass ---->(+) Zooplankton

On the other hand, by negatively affecting the minnows population, basses will be indirectly and positively influencing the zooplankton population. This fact is because the more minnows basses eat, the fewer fishes there will be to prey on zooplankton. So the invertebrate will have enough time to recover its population size. The predation pressure acting on zooplankton will decrease thanks to the action of basses preying on minnows.

Here there is a natural equilibrium between the three species. It is a representation of typical cycle predator-prey.

Minnows prey on zooplankton. Minnows population grows, and the zooplankton population decreases.As the minnow population grows, there is more available food for basses, who eat more minnows. As the bass population increases, the minnows population decreases in size. And as there are fewer minnows, the zooplankton population will increase. If minnows decrease in number, basses will decrease in number too. Minnows will not be severely affected by basses, so their population increases again. As zooplankton also increased, there will be more available food for minnows that will keep reproducing. And the more minnows there are, the more available food for basses there will be. And basses population will increase again. So you can see that zooplankton is indirectly and positively affecting the basses population too (not shown in images).

In a separate location, take notes from the sources you've identified. The notes will provide details for your presentation
While taking notes, you may want to use these reading strategies C. Write down one or two pieces of information per
topic that you intend to use in your presentation.
Topic
Chemical reactions of fossil fuels
Difference between biomass and fossil fuels
Comparing biomass with other renewable
energy sources
Making energy choices locally

Answers

Answer:

CH4 + 2O2 -----> CO2 + 2H2O.

Explanation:

CH4 + 2O2 -----> CO2 + 2H2O is the chemical reaction of burning of fossil fuels. The main difference between biomass and fossil fuels is that biomass is biological material which can be derived from living whereas Fossil fuel such as coal, oil and gas are derived from biological material. Biomass is renewable because a new crop can be grown after each harvest, and biomass is a low carbon fuel. Biomass is renewable just like other renewable energy sources because both can be used again and again. The energy choices locally occurs according to the availability of energy resources in that locality.

Which of the following best describes a parasite?
a. An organism that hunts and kills other organisms

b. An organism that lives in or on another organism and uses it in some way

C. An organism that helps other organisms by providing them with nutrients

d. None of the above

Answers

The answer is B, I hope you do great on the assignment!

The thyroid gland produces a substance
containing iodine that helps regulate growth.
Goiter is an enlargement of the thyroid gland
caused by a lack of iodine in the diet. Goiter
is uncommon in coastal areas where people
eat seafood that contains a form of iodine.
It is more common inland, where people
eat food that has been grown in soil
lacking iodine.
Which phrase restates the cause of the
disease goiter, as given in the passage?
(1) eating too much fish
(2) eating too little of foods high in iodine
(3) exposure to a bacterium found in iodine
(4) fertilizing the soil with iodine
(5) inheriting a defective thyroid gene

Answers

Answer:

eating too little of foods high in iodine

Explanation:

Iodine deficiency lead to goiter that is why it is necessary to consume food rich in iodine like taking iodized salt or sea foods which prevent this. This lead to goiter because, your thyroid gland need iodine to repair tissues, produce thyroid hormones, aid metabolism and for proper growth,therefore deficiency of it will result in non of the following functions.

What happens to water on Earth’s surface as it is heated by the sun? What is this process called?


Explain your answer.

Answers

Answer:

As the water is heated by the sun, water vapor is created and released into the air (process is called evaporation)

Explanation:

The heat coming from the sun heats up the water droplets and turns those droplets from a liquid into a gas (water vapor)

If u and y are two variables such that
du
= 4v - 3. and u = 5 when v = 2,
du/dv
find u in terms of v.​

Answers

Answer:

5

Explanation:

The value of u is 5 in term of v by putting the value o v which is 2 in the formula that is du = 4v - 3. Putting the value of v = 2 we get, du  = 4(2) - 3. --- du = 8 - 3. ---- du  = 5. we are able to solve the question due to the presence of v value. If the v value is not present then we can't find the value of u in term of v. So we can conclude that the value of u in term of v is 5 if we put the value of v in the formula.

Which of the following is a disadvantage of hydrogen fuel?

Answers

Answer:

The answer is A. It is expensive to produce and transport

Explanation:

In guinea pigs, the short hair trait is dominant. If two heterozygous guinea pigs were crossed, what would be the phenotypes of the offspring? *
A. 100percent short hair
B. 75 percent short and 25 percent long.
C. 50percent short and 50 percent long
D. 25 percent short and 75 percent long​

Answers

Answer is B. Best way to check is make a square

A young woman was going on a walk through the woods when she felt a mosquito bite her. She has a history of large local reactions to mosquito bites in the past. The reactions have been so severe she has even been skin tested, but the test came out negative. After the most recent bite, she went back to the doctor complaining of swelling at the site, dizziness, headache, and nausea. Benadryl is able to help relieve some symptoms. The doctor decides to try a blood test (i.e. ELISA based test) instead to test for allergies.

Required:
a. What is an ELISA test and how is it done?
b. Which antibody would they be looking for specific in this case?
c. The doctors also ran an SDS-PAGE test using the patient's serum mixed with two different antigens: lane 1 - bee venom; lane 2 - mosquito venom. What do the results show regarding the patient’s allergies?
d. Why are there two dark blue bands in lane 2?
e. Describe what happens during this specific type of allergic reaction.

Answers

Answer: uhh

Explanation: i need points

A certain lake has a volume of about 480 m³. If the total population of freshwater shrimp in the lake is 600 million, what is the population density of the shrimp in that lake?

Answers

Answer:

1,250,000 shrimps per m³

Explanation:

Population density refers to how dense an area is per unit area/volume, and it is calculated by dividing the number of individuals in that area (population) by the area/volume of that area. That is;

Population density = population (N)/volume

According to this question, a certain lake has a volume of about 480 m³ that harbors a 600 million of freshwater shrimp. This means that;

Population density of the lake = population of shrimps ÷ volume of lake

Population density = 600,000,000 ÷ 480

Population density = 1,250,000 shrimps per m³

13) On Earth, water vapor occurs in the state of matter. a.) Solid Ob.) Liquid C.) Gas d.) Plasma

Answers

Answer:

Gas

Explanation:

Because when we heat water water vapour will come in the gas form

Answer:

The answer is gas

Explanation:

Sorry I don't really know how to explain, when water vapor occurs, the water rise up to the sky in the form of gas.

Hope this will help❤️

6
6.
Which behavior can help animals establish a
territory?
A. A caribou herd migrates seasonally.
B.
A scent trail leads ants to a food source.
C.
A wolf pack howls to warn other wolves to
stay away.
D.
A honeybee society divides the work between
its members.

Answers

C bc wolf pack letting everybody know it’s their territory

what is the role of microorganisms in food preservation?​

Answers

Answer:

Preservation usually involves preventing the growth of bacteria, fungi (such as yeasts), and other microorganisms.

Explanation:

Nature uses microorganisms to carry out fermentation processes, and for thousands of years mankind has used yeasts, molds and bacteria to make food products such as bread, beer, wine, vinegar, yogurt and cheese, as well as fermented fish, meat and vegetables reduce food spoilage.

^Hope it helps, Hazel^

You eat at chips and salsa at Qdoba. All that salt causes your cells to lose water through
osmosis. Your hypothalamus senses that your cells are shrinking
and sends a message to the
pituitary to release antidiuretic hormone. ADH sends a message to the kidneys to stop
releasing water in the urine. You also start to feel thirsty. The result is that your cell's water
level increases back to normal. This is an example of


A: Negative feedback
B: Positive feedback

Answers

Answer:

B

Explanation:

this is going to cause your body cells to hold water

Hi can you help please please thank you

Answers

Answer:

10 N

Explanation:

in all honesty I'm unsure but I think it is 10N because 20N of force is pulling on way but 10N is pulling the other way so 20-10 is 10N and that's the answ

PLEASE HELP I HAVE AN EXAM !!!
With the aid of a diagram, show how secretion of cortisol is regulated at the primary, secondary, and tertiary levels

P.S. If you do not know the answer, could you please provide the link for the site where there is an answer. Appreciate your help !!!

Answers

Explanation:

Cortisol secretion is regulated by the hypothalamic hormone, CRH, and the pituitary hormone, ACTH, in the hypothalamus-pituitary-adrenal axis. Cortisol is known as a stress hormone involved in the response to physical and/or emotional stress.

Which of the karyotypes shown is from a normal female?

Answers

Answer:

Karyotype C is from a normal female

Karyotype C is the normal female

AB blood type illustrates the principle of

Answers

The AB blood type has both A and B alleles.This illustrates principle of co-dominance

1. Some individuals of a population or species are better suited to
a. evolve than other individuals.
b. survive and reproduce.
c. become extinct.
d. achieve punctuated equilibrium.

Answers

Answer:

I believe it's b. survive and reproduce.

Question 9
O Mark this question
Being left-handed (represented as I) is a recessive trait. Jim and Suzie are both left-handed.
If they have a child, which of the following could be his/her genotype?
O LL
O Either Ll or II
OLI
II
SAVE AND CONTINUE

Answers

Their genotype could either be Ll or ll because they can either get one dominant and one recessive gene or they could get ll which is two recessive genes!

The correct option is (2) either LI or II .

The term genotype refers to the genetic component of a trait.

The phenotype is the genotype's visible manifestation.Due to the fact that an existing DNA strand is used to make a new strand, DNA replication is known as semiconservative.

What does the term genotype mean?The type of variant present at a specific locus (i.e., region) in the genome is scored by what is known as a genotype. Symbols can be used to symbolize it.There are three different genotype types: hetrozygous, homozygous recessive, and homozygous dominant.Learn more about genotype brainly.com/question/20730322

#SPJ2

A student is planning an investigation of the effect of population size on an ecosystem. She plans to use a five-gallon fish tank with ten goldfish for her investigation. How should the student perform her investigation? A. She should limit the amount of food given and observe the results. B. She should add a predator fish to the tank and observe the results C. She should continue to add more goldfish to the tank and observe the results D. She should change the temperature of the water in the tank and observe the results.​

Answers

Answer:

C

Explanation:

Because the student is observing the effects on the population, so it would make more sense to add more fish to the tanks to see how they would get effected.

A student is planning an investigation of the effect of population size on an ecosystem, so she should continue to add more goldfish to the tank and observe the results, as in Option C.

What is the population size of an ecosystem?

In the student's investigation, the goal is to observe the effect of population size on an ecosystem using a fish tank with goldfish. The student has already decided to use a five-gallon fish tank with ten goldfish for the experiment, so by adding more goldfish to the tank, the student can observe the effect of population size on the ecosystem. As the population of goldfish increases, the competition for resources such as food, oxygen, and space will also increase. This can lead to changes in behavior.

Hence, she should continue to add more goldfish to the tank and observe the results, as in Option C.

Learn more about the population size of an ecosystem here.

https://brainly.com/question/28109247

#SPJ2

PLZ HELP ASAP (BRAINLIEST INCLUDED)

Which of the following are seen when the solar winds collide with Earth's atmosphere?

Auroras
Lunar eclipses
Solar eclipses
Tides

Answers

Answer:

Auroras

Explanation:

Hope this helped :)

Answer:

Auroras

Explanation:

Auroras often form near Earth's north and south magnetic poles, but sometimes they can also occur near the equator. Their position all depends on solar winds. The stronger the solar wind is, the bigger and brighter the aurora is.

hope this helps ♥

everyone

plz do j,oin

through
g,oogle
m,eet


jtoessthpr​

Answers

everyone

plz do j,oin

through

g,oogle

m,eet

jtoessthpr

[tex] \huge{괜찮아}[/tex]

Rocks that are formed from sediment that has been compressed over time and becomes solid are called ______________ rocks *
1 point
igneous
metamorphic
sedimentary
Plzzzzzz help ME NEED IT BY TODAYYY

Answers

Answer:

Metamorphic, :b

Explanation:

Lol, hope this helps ;B

give This question a shot and I’ll give you brainliest no links posted on My question or I will report you

Answers

Answer:

B) 113

Explanation:

Answer:

If we convert 45 degrees Celcius to Farenheit, we will get 113 degrees Farenheit

On the planet Feline, there are only cats. For one particular phenotype, Myles, there are two manifestations: either the cats like jazz music (the wild type phenotype) or they do not (the mutant phenotype). Under the assumption this phenotype is determined by a single gene with two alleles, and given that a mating of two wild-type cats produces the following number of offspring: Female, wild-type: 341; Male, wild-type: 369; Female, Myles: 121; Male, Myles: 123. The mode of inheritance most consistent with these data is:________
A. Autosomal dominant.
B. Two-locus inheritance.
C. Sex-linked recessive.
D. Autosomal recessive.
E. Two-locus dominant.

Answers

Answer:

D. Autosomal recessive.

Explanation:

Available data:

One diallelic gene codes for the phenotype MylesOne allele expresses the wild type phenotype → like jazz musicThe other allele expresses the mutated phenotype → don´t like jazzCross: Between two wild catsF1) Female, wild-type: 341; Male, wild-type: 369; Female, Myles: 121; Male, Myles: 123    

Autosomal recessive inheritance characterizes because the mutated gene locates in one of the 22 non-sexual, somatic chromosomes. To express the phenotype, an individual must carry two copies of the mutated gene. For this reason, the recessive allele must be present in both the male/father and the female/mother.  

In the exposed example, the numbers among the progeny are 341:369:121:123. These values approximate a phenotypic ratio 3:1 for females, and for males. This ratio suggests that the cross was performed between two heterozygous individuals.

Let us name the alleles M and m, dominant and recessive respectively. The cross would be like follows

Cross:  wild-type male    x     wild-type female

Parentals)   Mm XY         x                MmXX

Gametes)  MX, MY, mX, mY       MX, MX, mX, mX

Punnett Square)     MX         MX         mX           mX

                  MX    MMXX    MMXX    MmXX     MmXX

                  mX    MmXX    MmXX    mmXX     mmXX

                  MY    MMXY    MMXY     MmXY     MmXY

                  mY    MmXY    MmXY     mmXY     mmXY

F1) Whole progeny

12/16 = 3/4 = 75% Wild-type cats → MMXX + MmXX + MMXY + MmXY4/16 = 1/4 = 25% Mutated cats → mmXX + mmXY

     Females

6/8 = 3/4 = 75% Wild-type cats → MMXX + MmXX 2/8 = 1/4 = 25% Mutated cats → mmXX

     Males

6/8 = 3/4 = 75% Wild-type cats → MMXY + MmXY2/8 = 1/4 = 25% Mutated cats → mmXY

The oxygen cycle is closely related to which other cycle?

A. The carbon cycle
B. The nitrogen cycle
C. The phosphorus cycle

Answers

Answer:

A

Explanation:

that what i think

i don't know if I'm right

explica la relación entre el desarrollo de la resistencia a un insecticia entre una especie de insectos y la adaptación​

Answers

Answer:explains the relationship between the development of resistance to an insecticia between a species of insects and the adaptation

Explanation:

Which of the following thrive in tropical areas and absorb light?



Greener Air Plants

Grayer Air Plants

Answers

Answer: Greener air Plants

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Many years ago a scientist Group E plants that produce wrinkled peas the peas from the plants produce new plants that also produce wrinkled peas the scientist concluded that something in the para plan will be transmitted to the next generation this discovery is known as Trevor is walking at the rate of 6 kilometers per hour. How many meters does Trevor walk in a minute? In the late 1950's, scientists in South America imported African honeybees to breed with honeybees native to the region. The scientists hoped the hybrid bees would produce more honey. Some of the African bees escaped their hives and began breeding in the wild in large numbers. The African bees have outcompeted native bees for resources, decreasing the number of native honeybees. The African bees that have escaped into the environment are: a. a climax community b. an invasive species c. a pioneer species d. an ecological succesion ASAP pls solve quick and show work 0.5x + 2 = 10 What are the coordinates of the point labeled A in the graph shown above?Question 4 options:A) (2, 4)B) (2, 2)C) (2, 2)D) (2, 2) 9. Lobbying for or against trade restrictions Trade restrictions affect the overall welfare of an economy because they change the price consumers pay for a good and the quantity produced and consumed domestically. Trade restrictions, such as tariffs, usually benefit domestic and hurt domestic because they the domestic price of a good. True or False: Producers find it difficult to exert the political influence needed to establish trade restrictions because the benefits to producers are very small and widely dispersed, which makes it difficult for producers to organize. True False The angle of elevation to the top of a Building in Seattle is found to be 5 degrees from the ground at adistance of 1 mile from the base of the building. Using this information, find the height of the building. College Math!!! I did the first half of the question, I just need the bottom half of it. 4NH3 + 502 - 6H20 + 4NO How many grams of O2 are required to produce 0.3 mol of H20?4NH3 + 5O2 --> 6H2O + 4NOHow mant grams of NO are produced from 1.55 mol of NH3? 4NH3 + 5O2 --> 6H2O + 4NOHow many grams of NO is produced if 12g of 02 is combined with ammonia? Lash Corporation has the following sales budget for the last half of 2000:May $164,000 June $145,000 July $206,000 August $181,000September 168,000 October 203,000 November 209,000 December 185,000 Sales are immediately due, however the cash collection of sales, historically, has been as follows: 55% of sales collected in the month of sale, 35% of sales collected in the month following the sale, 7% of sales collected in the second month following the sale, and 3% of sales are uncollectible.Required:a. What are the expected cash collections in September?b. What is acciounts receivable at September 30? Economic problems can often contribute to social problems. How did this happen during the Great Depression? Plz help this is due today AND NO LINKS OR GROSS PICTURES OR I REPORT. PLZZ HELP MEEE YOOOWhat do bones some unformed bones in the hands start as?1.blood cells2.veins3.tissue4.cartilage simplify (4x-2)+3(x+7) Describe how the branches of psychology interact What themes are revealed through the descriptions and interactions of Richard Kennedy and Samuel Ferguson? Select all that apply. A True friends are willing to forgive past mistakes. B Even the closest friends face challenges in their relationship. C Even the best friendships can fade over time. D Good friends always agree about important issues. E Friendship is more important than success. F Close friendships can develop between very different people. I'm not sure at all how to do this The foxglove plant, seen in the illustration, produces very toxic chemicals that can cause serious illness and death in anyorganisms that eats the plant. Why do scientists think the foxglove plant evolved such powerfur poisonsAB)The poisons function to kill invading bacteria and other pathogens thatmay harm the plant.The poisons maximize the efficiency of the photosynthetla process.allowing the plant to make more foodThe polsons discourage any animal from eating the plant preventingdamage to the plant from herbivores.D)Poisons are likely to be the product of genetia mutations in the DNA of theplant and do not have any useful function Sets A and I are defined as follows.A={-5, -2,0,2,5}I={-5, -1,1,5,8}Find the union of A and I. a) who are some group of people who migrate to St.Thomas? b) what are some of the reasons that people migrate here? c) what area on St.Thomas would be best to create a recreational park? give your reason.