Many eukaryotic genes can be placed into bacteria and expressed in bacterial cells. However, some modifications must be made for this to work successfully. What do you predict is a primary issue with expressing eukaryotic genes in bacterial cells?.

Answers

Answer 1

Because E. coli is a prokaryote and lacks the membrane-bound nucleus (and other organelles) present in eukaryotic cells, utilizing it to express eukaryotic genes has several drawbacks.

The pre-introns mRNAs won't be cut out, which will cause the synthesis of an aberrant protein. In bacteria, the sequences needed for effective translation initiation do not exist. Due to the cells' inability to carry out the necessary post-translational modifications or molecular folding, multi-domain eukaryotic proteins produced in bacteria frequently lack functionality.

Finding the best combination of information sources extrinsic and internal to the genome of interest is one of the main challenges of gene prediction in eukaryotes. RNA transcripts and cross-species proteins produced from annotated genomes may both carry external information.

As a result, we can state that E. coli is a prokaryote and does not have a membrane-bound nucleus or other eukaryotic cell organelles, using it to express eukaryotic genes has a number of disadvantages.

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Related Questions

Which of the following is an effect of opsonization?
A) increased adherence of phagocytes to microorganisms
B) increased margination of phagocytes
C) increased diapedesis of phagocytes
D) inflammation
E) cytolysis

Answers

Opsonization has the effect of increasing the phagocytes' adhesion to bacteria.

What is example of opsonization?

Examples of opsonization include antibody molecules like the IgM, which can activate the complement system to make antigens more phagocytic-susceptible. Opsonization can enhance antibody-dependent cell-mediated cytotoxicity in addition to phagocytosis.

What is opsonization and why is it important?

Opsonization is a crucial step in innate immunity that prepared particles or complexes for easy absorption by phagocytic cells. Particle coated with particular serum proteins, called ensuring a high level, adhere ardently to phagocytic cells that induce ingestion.

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depressants are ________ of the gaba neurotransmitter, which has a quieting effect on the brain.

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Depressants are agonists of the GABA neurotransmitter, which has a quieting effect on the brain.

Sedatives, tranquilizers, and hypnotics are examples of Central Nervous System (CNS) depressants. These medications have the ability to slow brain activity, making them useful for treating anxiety, panic attacks, acute stress reactions, and sleep disorders. Arousal and stimulation are reduced by depressant substances. They have an effect on the central nervous system, slowing messages between the brain and the body.

Gamma-aminobutyric acid (GABA) is an amino acid that serves as the central nervous system's primary inhibitory neurotransmitter. It works by inhibiting nerve transmission to reduce neuronal excitability. It slows down your brain by blocking specific central nervous system signals. GABA is known to have a calming effect.

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The rate at which a specific allele for red flower color appears within a population is known as its.

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The rate at which a specific allele for red flower color appears within a population is known as its  allele frequency .

Phenotype frequency: what is it?

Phenotype is the term used to describe how DNA manifests physically. The phenotypic ratio only describes the physical traits, or phenotypes, that are present in a population. The term "genotype" broadly refers to an organism's genetic make-up, or, to put it another way, the entire collection of its genes. The phrase can also be used more specifically to describe to a gene's alleles, or variant versions, that an organism carries.

The frequency of a particular allele in a population is known as the allele frequency (or gene frequency). The term "evolution" in population genetics refers to a shift in an allele's frequency within a population.

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proper segregation of plasmids into daughter cells may incorporate which of the following strategies?
a. Random partitioning due to high copy number
b. Polymerization of a filament that binds to, and physically separates plasmids
c. Post-segregational killing via toxin / antitoxin system

Answers

Proper segregation of plasmids into daughter cells may incorporate which of the following strategies Polymerization of a filament that binds to, and physically separates plasmids.

A plasmid is a small, circular, double-stranded DNA molecule that is distinct from a cell's chromosomal DNA. Plasmids certainly exist in bacterial cells, and in addition they occur in a few eukaryotes. often, the genes carried in plasmids offer micro organism with genetic advantages, which includes antibiotic resistance.

A plasmid is a small circular DNA molecule determined in micro organism and some other microscopic organisms. Plasmids are physically separate from chromosomal DNA and mirror independently.

Plasmids are critical for bacterial evolution and variation to the changing environment, as they carry genes which bring beneficial trends for the bacterial cellular. exclusive kinds of plasmids can coexist in a single bacterial mobile.

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What is the relationship between the cell size and the cell nutrient absorption rate?

Answers

The smaller the cell, the more favorable the ratio of volume to surface area for material exchange with the atmosphere is done by diffusion. Although surface area determines the rate at which nutrients are taken in or excreted, the volume determines how much enters and leaves the cell.


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A brown-eyed man whose mother was blue-eyed married a brown-eyed woman whose
father had blue eyes. Assume brown to be dominant over blue. What is the probability
that the couple will have a blue-eyed child?

Answers

The probability will be 0.25 or 25%

explain the connection between dna, rna, proteins, and traits and describe how transcription and translation

Answers

RNA is produced using the DNA code. The polypeptide chain of a protein is built using RNA as a template. Thus, RNA follows DNA, which follows proteins.

How do DNA and RNA replication and translation connect to one another?

According to the Central Dogma of Molecular Biology, proteins are made from DNA rather than RNA. Transcription and translation are the processes by which DNA is converted into RNA and used to make proteins, respectively.

What happens when transcription occurs to DNA and RNA?

A single-stranded RNA molecule is created during transcription from a piece of the double-stranded DNA template. In some instances, the RNA molecule itself performs a crucial task for the cell.

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Crick and brenner performed a set of experiments with viral dna designed to determine the nature of the genetic code. What were their conclusions?.

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The genetic code has no punctuation as well as the genetic code is read in increments of three nucleotides were the conclusions of Crick and benner.

With the use of straightforward genetic experiments, Francis Crick, Sydney Brenner, and two Cambridge colleagues showed that the genetic code was very probably built on groups of three nucleotides in a Nature paper that was published in the year 2000. A live cell's ability to convert the data stored in genetic material into proteins is governed by a set of principles known as the genetic code.

Numerous scientists initially questioned whether DNA and RNA actually conveyed the genetic code due to the relative simplicity of nucleic acids. Long before it was feasible to sequence nucleic acids, Crick and Brenner demonstrated using just the tools of genetics that the genetic code was almost certainly based on groups of three nucleotides.

 

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the brain scans of people with amnesia are most likely to show damage to the responses hippocampus hippocampus hypothalamus hypothalamus medulla medulla reticular formation reticular formation cerebellum

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The brain scans of people with amnesia are most likely to show damage to the hippocampus.

What is amnesia?

Amnesia is the loss of memories, including details, knowledge, and experiences. Although losing your identity is a frequent plot technique in movies and television, this isn't usually the case when someone has amnesia in real life. Instead, those who have amnesia, also known as amnestic syndrome, typically are aware of who they are.

What do you understand by hippocampus?

In the brains of humans and other vertebrates, the hippocampus plays a significant role. Hippocampi are located on each side of the brain in mammals like humans. The hippocampus is a component of the limbic system and is crucial for spatial memory, which is necessary for navigation, and for transferring information from short-term to long-term memory. In both humans and primates, the hippocampus is found in the allocortex and projects neuronal fibres into the neocortex. All vertebrates have the hippocampus, which is the medial pallium. The dentate gyrus and the hippocampus proper, also known as Ammon's horn, are its two primary interconnecting components in humans.

Thus from above conclusion we can say that the brain scans of people with amnesia are most likely to show damage to the hippocampus.

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functions can be called from statements in the body of a loop and loops can be called from within the body of a function.

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Answer:  Functions can be called from statements in the body of a loop and loops can be called from within the body of a function. In a nested loop, the inner loop goes through all of its iterations for each iteration of the outer loop.

Explanation:

in order to identify a species by its scat, which of the following can be utilized to help identify it? Select all that apply

size of scat
smell of scat
texture of scat
color & shape of scat

Answers

Size, smell texture and color & shape of scat are all utilized to help identify species.

Protein and DNA analysis are the most commonly used methods for species identification, with protein characterization performed using immunological, electrophoretical, and chromatographic techniques. Historically, species identification has been based on morphological data and implemented in dichotomous identification keys. Specimens can also be identified through sequence similarity in taxonomically curated sequence databases, thanks to easy access to increasingly affordable DNA sequencing.

Species identification is critical in food authentication for a variety of reasons, including food safety, food selection, and religious practices. The presence of undeclared protein may have serious health consequences for people who are allergic to it.

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If a test sample burns completely in a calorimetry experiment, it has reached A. partial degradation. C. complete degradation. B. reversible degradation. D. measurable degradation.​

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If a test sample burns completely in a calorimetry experiment, it has reached: C. complete degradation.

What is a calorimeter?

A calorimeter can be defined as a scientific instrument or device that is designed and developed for taking the measurement of the quantity of heat involved in chemical reactions or other heat-related processes, especially by taking the measurement of the temperature of the materials surrounding the process.

In Chemistry, complete degradation is a terminology which is used by scientists and researchers to describe a test sample that burns completely in a calorimetry experiment such as the burning of water (H₂O).

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cave art by early humans recognized the existence of the major signs of inflammation. the most inclusive set of symptoms of inflammation that might appear in such early human art is?

a. antibody-producing cells.
b. swelling and pain only.
c. swelling, heat, redness, and pain.
d. pain and whitening of the surrounding tissue.

Answers

Those are the main symptoms of information that might appear in such early human art. so the correct option is C swelling, heat, redness, and pain.

Blood flow is enhanced, which results in redness and heat. The increased flow of fluid and white blood cells into the damaged area causes swelling. Pain is brought on by the release of chemicals and the compression of nerves in the injured area. White blood cells in your body release substances during inflammation to defend your body from foreign intruders. As a result, the area of injury or illness receives increased blood flow. Warmth and redness may result from it. Some of the chemicals lead to swelling in your tissues by causing fluid to flow into them.

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How have human impacted the ecosystem? ​

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Answer:

Humans have impacted the ecosystem greater than any other organism on earth. Human life has done tremendous amount of damage. Humans have caused air pollution threw cars, buses/automobiles. The use of powerplants and production line seriously effects the eco system to a greater extent. I u have heard u leave a carbon footprint wherever you go which leaves a big impact on the ecoystem.

Explanation:

organisms that use co2 as their source of carbon are called multiple choice organotrophs. heterotrophs. autotrophs. chemotrophs.

Answers

Organisms that convert inorganic carbon dioxide (CO2) into organic carbon compounds are autotrophs.

Autotrophic organisms derive this essential element from carbon dioxide.

Mostly, Autotrophs are responsible for capturing the carbon dioxide from the air and use them in making organic compounds such as glucose. On the other hand  Heterotrophs, or the one who feeds on others for example humans, consume the organic molecules, and the organic carbon is passed through food chains and webs.

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what makes agglutination by antibodies possible?

Answers

Answer:

The presence of agglutinins is what makes agglutination by antibodies possible.

Explanation:

The unique binding characteristics of antibodies and their ability to cross-link antigens enable agglutination by antibodies.

The immune system reacts to the presence of foreign substances known as antigens by producing Y-shaped proteins called antibodies. Agglutination occurs when antibodies recognize and bind to multiple antigens present on the surface of cells or particles. Because each antibody molecule has two antigen-binding sites, it can bind to two different antigens at once. This binding leads to the deposition or aggregation of cells or particles, which form cross-links between the antigens.

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Dna sequence differences, metabolism, and cell structures viewed under the microscope are ways that scientists identify and classify ______ organisms.

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DNA sequence differences, metabolism, and cell structures viewed under the microscope are ways that scientists identify and classify Prokaryotic organisms.

Prokaryotes are organisms whose cells lack a nucleus and other organelles. Prokaryotes are divided into two wonderful organizations: the microorganism and the archaea, which scientists believe have particular evolutionary lineages. maximum prokaryotes are small, unmarried-celled organisms that have a tremendously simple shape.

A prokaryote is an unmarried-celled organism that lacks a nucleus and other membrane-bound organelles. The word prokaryote comes from the Greek and . within the two-empire gadget springing up from the paintings of Chatton, prokaryotes have been categorized within the empire Prokaryote.

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which of the following antimicrobial medications inhibit nucleic acid synthesis in bacteria?check all that applyrifamycinsrifamycinsmetronidazolemetronidazolechloramphenicolchloramphenicolpolymyxin bpolymyxin bfluoroquinolonesfluoroquinolones

Answers

Rifamycins, sulfamides, and diaminopyridines are the Antimicrobial medications.

What are antimicrobial medications?

Antibiotics, antivirals, antifungals, and antiparasitics are medications used to prevent and treat infections in people, animals, and plants. Penicillin, for instance, is produced by a variety of fungi in the genus Penicillium, and vancomycin is produced by a bacterium known as Amycolatopsis orientalis; as a result, both are true antibiotics, in contrast to ciprofloxacin and linezolid, which are synthetic compounds and are therefore technically antimicrobials.

Using antibiotics, bacterial illnesses are treated. Beta-lactams, macrolides, quinolones, tetracyclines, or aminoglycosides are the general categories under which antibiotics fall.

To treat infections brought on by gram-negative rods, most notably Pseudomonas species, gentamicin and tobramycin are most frequently used and the main medications. The aminoglycosides are usually bactericidal antibiotics, despite the fact that most bacteriostatic drugs that block protein synthesis are bacteriostatic.

The strongest antibiotic is called vancomycin 3.0.created to date. Meningitis caused by methicillin-resistant Staphylococcus aureus, endocarditis, joint infections, bloodstream infections, and skin infections are among the problems it is used to treat.

Rifamycins, sulfamides, and diaminopyridines are a few antimicrobials that work by preventing the synthesis of nucleic acids, altering their structure (quinolones, coumarins), or inflicting irreversible damages (nitroimidazoles, nitrofurans)

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Under similar warm, moist climatic conditions, why would basalt and gabbro generally have higher chemical weathering rates than rhyolite and granite?.

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Under similar warm, moist climatic conditions, basalt and gabbro generally have higher chemical weathering rates than rhyolite and granite because the ferromagnesian minerals in the gabbro and basalt are subject to oxidation and chemical breakdown.

Weathering is the process of breakdown of rocks and minerals due to the continuous contact with air, eater or some kind of biological agent. This process is the reason for the presence of several types of landforms on the Earth.

Ferromagnesian minerals are actually the silicate minerals that contain the cations of iron and magnesium. The examples of ferromagnesian minerals are:  Olivine, Pyroxene, Amphibole, Biotite, etc.

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GIVING BRAINLIST + THIS QUESTION WORTH 50 POINTS
Students in Mr. Smith's science class were taking random samples throughout the schoolyard for population analysis. One easy-to-locate organism that they counted was the earthworm, Lumbricus terrestris. Their data is displayed above. Several students were concerned about the count in plot C, as it was much lower than the other plots. Sandra suggested that they recount, but this time, count the earthworms over an extended period of time. Sam commented that it would skew the results in the other direction. He added that this was a random sample, and plot C was in sandy soil near the parking lot. What comment would you make concerning the students experimental design? Q: A: A) I would omit plot C from the data and consider it as an error. B) I agree with Sandra and count the earthworms in plot C over an extended period of time. C) I agree with Sam. The plot selection was random and earthworms do not typically live in sandy, dry soil. D) I would omit the idea of random selection of plots and purposely select plots I thought would be typical of earthworm habitats. Hint Regular Calc Scientific Calc

Answers

Answer:

i will comment (c) because what he said there was kinda right because Earth worms can't be found in dry soil

Which of the following best describes a supporting ecological service provided by wetlands?

a. Wetlands serve as a source of aesthetic inspiration for art and literature.
b. Wetlands provide opportunities for ecotourism, which provides revenue to the local economy.
c. Wetlands serve as a major reservoir for nitrogen in the nitrogen cycle.
d. Wetlands provide a habitat for a wide variety of organisms.

Answers

D. Wetlands provide a habitat for a wide variety of organisms.

Important  purposes of wetlands are Water purification , protection from flood. Economic benefits to wetlands are Improved water quality, flood control, wildlife and fisheries habitat, and recreational opportunities .

Wet are very important source they are used to store water to supply during dry periods , helps in flood management, control soil erosion .They are also important for providing shelter to fishes and other water creature . They can be helpful for aquatic plants and animals. These valuable and diverse uses and functions are unique characteristics of wet lands.

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What substance is the usable source of the energy that a cell uses for the synthesis of materials?.

Answers

The substance that is the usable source of the energy that a cell uses for the synthesis of materials is ATP.

The chemical substance that toils as the currency of energy in a cell is adenosine triphosphate (ATP). ATP is attributed to as currency because it can be used to make chemical reactions happen. The more energy needed for a chemical reaction, the more ATP molecules must be spent.

Normally, all forms of life use ATP, a closely universal molecule of energy transfer. The energy freed during catabolic reactions is stored in ATP molecules. In addition, the energy restricted in anabolic reactions (such as photosynthesis) is trapped in ATP molecules.

The adenosine triphosphate enzyme fulfills the breakdown of an ATP molecule.

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which of the following occurred first in the history of life? answer evolution of multicellular organisms evolution of eukaryotic organisms plants and fungi colonizing land the beginning of atmospheric accumulation of o2

Answers

The following which occurred first in the history of life is the beginning of atmospheric accumulation of O₂ and is denoted as option D.

What is Oxygen?

This is referred to as a chemical element which has an atomic number of 8 and is also an important gas which is used during the respiration process kin organisms. Plants give off this gas as a product of photosynthesis thereby making it available.

For organisms to survive.  there must be the atmospheric accumulation of oxygen gas which is why it occurred first around billions of years ago in the history of life.

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what does the observation that the alu transposon is limited to this clade reveal about its origin and method of spread among species?

Answers

This remark indicates that the ALU element originated within the commonplace ancestor of that clade and changed into then exceeded vertically to descendent species.

An Alu element (or surely, “Alu”) is a transposable detail, additionally called a “jumping gene.” Transposable elements are rare sequences of DNA that can pass (or transpose) themselves to new positions inside the genome of a single mobile. ALU factors are about 300 bases long and are located all throughout the human genome.

ALU factors are a sort of "jumping gene," or transposable element (TE), that exists only in primates. like every TEs, they may be discrete DNA sequences that circulate, or "jump," from one location at the genome to some other, from time to time placing copies of themselves at once into the center of protein-coding genes.

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which part of the brain influences the autonomic and hormonal responses via connections with the hypothalamus?

Answers

Paraventricular nucleus influences the autonomic and hormonal responses via connections with the hypothalamus.

Paraventricular nucleus is the nucleus present in the hypothalamus.  It is considered as one of the three zones of the hypothalamus. It is also responsible for the secretion of the hormone called oxytocin apart from regulating autonomic responses. The nucleus is actually a collection of several nuclei joined together with one motor output system.

Autonomic responses are the functions that are carried out on their own without our consciousness. These responses are digestion, pumping of heart, breathing, etc. The appearance of goosebumps is also an autonomic response.  

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a virus has a mass of and an oil tanker has a mass of . use this information to answer the questions below. be sure your answers have the correct number of significant digits.

Answers

5.4 x 10^12 mg is the mass of 1 mole of viruses.  6 moles of viruses have a mass equal to the mass of an oil tanker.

a. The mass of one mole of the virus is calculated by multiplying the mass of a single virus by Avogadro's number

=6.022 x 10^23

mass = (9.0 x 10^-12 mg)(6.022 x 10^23)

= 5.42 x 10^12 mg

5.4 x 10^12 mg is the mass of 1 mole of viruses.

b. The number of moles of the virus that will have the same mass as the oil tanker is calculated by dividing the mass of the oil tanker by the mass of one mole of the virus. Note that doing division, both would have to have the same units.

n = (3.0 x 10^7 kg) / (5.4 x 10^12 mg)(1 g/1000 mg)(1 kg/1000 g)

n = 6

6 moles of viruses have a mass equal to the mass of an oil tanker.

The complete question is:

A virus has a mass of ×9.010−12mg and an oil tanker has a mass of ×3.0107kg. use this information to answer the questions below. be sure your answers have the correct number of significant digits. what is the mass of 1 mole of viruses? g how many moles of viruses have a mass equal to the mass of an oil tanker? ×10

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Giant tube worms living near vents on the ocean floor do not have a functioning intestine. Instead they have a modified gut full of bacteria that provide the worms with carbohydrates. What is the energy that the bacteria use to make the carbohydrates?.

Answers

The energy that bacteria use to make carbohydrates by consuming small bacteria that, through a process known as chemosynthesis, obtain all of their energy from the substances in the water, because of the dark hue of the material that these hydrothermal vents emit, they are also referred to as "black smokers."

Prior to the discovery of odd hydrothermal vents by scientists examining the deep Pacific Ocean floor, the enormous tube worm, also known as Riftia pachyptila, was completely unknown to science. These vents circulate water that seeps down through fractures or faults in the rock and is heated by volcanic activity. The water that comes out of the vent is incredibly chemical and mineral-rich. Scientists were startled to see entire ecosystems of species surviving near these vents because this deadly soup of chemicals would be fatal to the majority of animals.

These critters were prospering in total darkness despite the water's temperature being close to boiling. Because they do not rely on sunlight for energy, the species that dwell close to these vents are distinct from all other living beings on earth. Instead, the several smaller species of tube worms that live in shallower waters are closely linked to the big tube worms.

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Suppoed the two thermometer on a ling pychrometer how almot identical reading. Wa the pychrometer more likely ued at a beach or in a deert in California? Explain you anwer

Answers

On a beach in Florida, the psychrometer was employed. A measurement device composed of a wet bulb thermometer is called a sling psychrometer.

Which thermometer is the most precise?

The most accurate approach to check body temp is with a digital thermometer. There are numerous varieties, many of which have multiple functions, including oral, rectal, & forehead variants. Following your choice of thermometer type, you can consider design, ancillary features, and cost.

What are some thermometer uses?

An device that measures temperature is a thermometer. It is able to gauge the temperature of solids like food, liquids like water, and gases like air. Celsius, Fahrenheit, & kelvin are the three most popular temperature measuring units.

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A cell with 18 chromosomes undergoes mitosis. How many daughter cells are created? [a] each daughter cell has [b] chromosomes.

Answers

When a cell with prior 18 chromosomes undergoes mitosis, it has 2 daughter cells with 18 chromosomes later.

Mitosis is one of the two types of cell division in which the cell which undergoes this process is divided into two daughter cells which in turn have the same number of chromosomes as the parent cell.

Here, we had one parent cell which means that after mitosis it is going to be divided into two daughter cells. The parent cell has 18 chromosomes at first and since this is the process of mitosis, the daughter cells are going to have 18 chromosomes each after undergoing mitosis as well.

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What structure in an interphase eukaryotic cell is the site of ribosomal rna transcription?.

Answers

The nucleolus is the largest nuclear organelle and is the primary site of ribosome subunit biogenesis in eukaryotic cells.
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