Weigh out the appropriate amount of HEPES sodium salt required to prepare 100 mL of a HEPES buffer that has pH 7.50 and is 90 mM in HEPES. FW for the HEPES sodium salt is 260.31. Transfer to a 150 mL beaker. Flowchart: Calculate the amount of HEPES sodium salt required to make the buffer.

Answers

Answer 1

Answer:

2.343 g of HEPES sodium salt is needed.

Explanation:

So, from the question above we have the following parameters which is going to help in solving this particular question.

The number of moles = 90mM, the pH = 7.50, FW for the HEPES sodium salt = 260.31, and the volume of HEPES sodium salt required = 100mL.

Therefore, the amount of HEPES sodium salt required to make the buffer = 260.31 × 9 × 0.001 = 2.343 grams of HEPES sodium salt is needed in 10mL.


Related Questions

. Predict the possible products for the following reaction and include
molecular, complete ionic, and net ionic equations.

NA2SO4 (aq) +γ (BrO3)2 (aq) --->

Answers

Answer:

Introduction

As a diligent student of chemistry, you will likely encounter tons of reactions that occur in aqueous solution (perhaps you are already drowning in them!). When ions are involved in a reaction, the equation for the reaction can be written with various levels of detail. Depending on which part of the reaction you are interested in, you might write a molecular, complete ionic, or net ionic equation.

Definitions of molecular, complete ionic, and net ionic equations

A molecular equation is sometimes simply called a balanced equation. In a molecular equation, any ionic compounds or acids are represented as neutral compounds using their chemical formulas. The state of each substance is indicated in parentheses after the formula. [Huh?]

Let's consider the reaction that occurs between \text{AgNO}_3AgNO

3

start text, A, g, N, O, end text, start subscript, 3, end subscript and \text{NaCl}NaClstart text, N, a, C, l, end text. When aqueous solutions of \text{AgNO}_3AgNO

3

start text, A, g, N, O, end text, start subscript, 3, end subscript and \text{NaCl}NaClstart text, N, a, C, l, end text are mixed, solid \text{AgCl}AgClstart text, A, g, C, l, end text and aqueous \text{NaNO}_3NaNO

3

start text, N, a, N, O, end text, start subscript, 3, end subscript are formed. Using this information, we can write a balanced molecular equation for the reaction:

\text{AgNO}_3(aq) + \text{NaCl}(aq) \rightarrow \text{AgCl}(s) + \text{NaNO}_3(aq)AgNO

3

(aq)+NaCl(aq)→AgCl(s)+NaNO

3

(aq)start text, A, g, N, O, end text, start subscript, 3, end subscript, left parenthesis, a, q, right parenthesis, plus, start text, N, a, C, l, end text, left parenthesis, a, q, right parenthesis, right arrow, start text, A, g, C, l, end text, left parenthesis, s, right parenthesis, plus, start text, N, a, N, O, end text, start subscript, 3, end subscript, left parenthesis, a, q, right parenthesis

[What kind of reaction is this?]

If we could zoom in on the contents of the reaction beaker, though, we wouldn't find actual molecules of \text{AgNO}_3AgNO

3

start text, A, g, N, O, end text, start subscript, 3, end subscript, \text{NaCl}NaClstart text, N, a, C, l, end text, or \text{NaNO}_3NaNO

3

start text, N, a, N, O, end text, start subscript, 3, end subscript. Since \text{AgNO}_3AgNO

3

start text, A, g, N, O, end text, start subscript, 3, end subscript, \text{NaCl}NaClstart text, N, a, C, l, end text, and \text{NaNO}_3NaNO

3

start text, N, a, N, O, end text, start subscript, 3, end subscript are soluble ionic compounds, they dissociate into their constituent ions in water. For example, \text{NaCl}NaClstart text, N, a, C, l, end text dissociates into one ion of \text{Na}^+Na

+

start text, N, a, end text, start superscript, plus, end superscript for every ion of \text{Cl}^-Cl

start text, C, l, end text, start superscript, minus, end superscript; these ions are stabilized by ion-dipole interactions with the surrounding water molecules. [I don't get it!]

Image of crystalline sodium chloride next to image of chloride and sodium ions dissociated in water. Each chloride ion is interacting with multiple water molecules through the positive dipole of the water, and each sodium ion is interacting with water molecules through the negative dipole of the water.

Image of crystalline sodium chloride next to image of chloride and sodium ions dissociated in water. Each chloride ion is interacting with multiple water molecules through the positive dipole of the water, and each sodium ion is interacting with water molecules through the negative dipole of the water.

Sodium chloride dissociates into sodium and chloride ions in water, and these ions become solvated by the highly polar water molecules. Image credit: "Salts: Figure 1" by OpenStax Anatomy and Physiology, CC-BY-NC-SA 4.0.

From the molecular formula, we can rewrite the soluble ionic compounds as dissociated ions to get the complete ionic equation:

\text{Ag}^+(aq) + \blueD{{\text{NO}_3}^-(aq)} + \maroonD{\text{Na}^+(aq)} + \text{Cl}^-(aq) \rightarrow \text{AgCl}(s) + \maroonD{\text{Na}^+(aq)} + \blueD{{\text{NO}_3}^- (aq)}Ag

+

(aq)+NO

3

 

(aq)+Na

+

(aq)+Cl

(aq)→AgCl(s)+Na

+

(aq)+NO

3

 

 

(aq)

+

Na

+

(aq)

+Cl

(aq)→AgCl(s)+

Na

+

(aq)

+

NO

3

 

(aq)

a. Use the balanced equation C3H8 + 5O2 -> 3CO2 + 4H2O to answer the following questions
i. How many moles of CO2 are produced from 5 moles 02? (1 point)

ii. How many grams of CO2 are produced from 5 moles O2? (2 points)
iii how many grams of CO2 are produced from 128.00g O2? (2 points)

Answers

Answer:

i. 3 moles.

ii. 132.03 grams.

iii. 105.6 grams.

Explanation:

Hello!

In this case, given the balanced chemical reaction, we can proceed as follows:

i. By starting with 5 moles of oxygen, via the 5:3 mole ratio we compute the produced moles of CO2:

[tex]n_{CO_2}=5molO_2*\frac{3molCO_2}{5molO_2} =3molCO_2[/tex]

ii. Now, since we have previously computed the moles of CO2 from the same moles of oxygen, by using its molar mass (44.02 g/mol), we obtain:

[tex]m_{CO_2}=3molCO_2*\frac{44.01gCO_2}{1molCO_2} =132.03gCO_2[/tex]

iii. Now, we need to combine the previously used two proportional factors for the calculation of the mass of CO2 from 128.00 grams of oxygen (molar mass 32.00 g/mol):

[tex]m_{CO_2}=128.00gO_2*\frac{1molO_2}{32.00gCO_2}*\frac{3molCO_2}{5molO_2}*\frac{44.01gCO_2}{1molCO_2}\\\\m_{CO_2}=105.6gCO_2[/tex]

Best regards!

what is a difference between water and wind

Answers

well i mean water is a liquid? —and wind is just.. air?

Answer:

You could have looked it up, but here ya go...

Explanation:

Wind is a type of dry deposition method. If original soil is eroded and wind exists, soil particles move via wind (sometimes it is called dust transport) and finally are deposited at final destination regions/areas. However, water erosion occurs when rainfall or hail occurs.

Hope this Helped! :)

Crude oil has many applications once it has been distilled at a refinery. According to the diagram, what process is used to distill crude oil into its various components to use energy distribution?

A) separation by density

B) separation by boiling points

C) separation by the porosity of the components

D) separation by the organic content or each component

Answers

Answer:

B) separation by boiling points

Explanation:

According to the diagram depicted, crude oil is separated into its different units using boiling points.

The boiling points of a compound is the temperature at which it begins to boil. The process of fractional distillation is used in the oil industry to separate crude oil into its components. When the boiling point of a oil fraction is reached, it becomes vaporized and rises within the column

Most organic compounds are made of just four elements.

What are they?

Answers

Answer:

hydrogen, carbon, oxygen and nitrogen

Explanation:

Most organic compounds are made up of the four elements: hydrogen, carbon, oxygen and nitrogen.

Most organic compounds are made of just four elements are  hydrogen, carbon, oxygen and nitrogen.

What do you mean by an organic compound ?

The term organic compound is defined as a substance that contains covalently - bonded carbon and hydrogen and often with other elements.

Benzoic acid, aromatic compounds, benzoic aldehyde, propanoic acid, butanoic acid, malonic acid, amines, heterocyclic compounds, VOC, benzoic acid, and diethyl malonate are some examples of organic compounds.

Carbohydrates, lipids, proteins, and nucleotides are all organic compounds that are required for human function. Because they contain both carbon and hydrogen, these compounds are classified as organic.

Thus, Most organic compounds are made of just four elements are  hydrogen, carbon, oxygen and nitrogen.

To learn more about the organic compound, follow the link;

https://brainly.com/question/5994723

#SPJ2

air is trasferred from 75 L tank where the pressure is 125 psi and the temperature is 288 K to a tire with a volume. of 6.1 L and a pressure of 25 psi. what is the new temperature ?

Answers

Answer:

4.7K

Explanation:

Given parameters:

Initial volume  = 75L

Initial pressure  = 125psi  

        to atm gives 8.5atm

Initial temperature  = 288K

New volume  = 6.1L

New pressure  = 25psi

        to atm gives 1.7atm

Unknown:

New temperature = ?

Solution:

To solve this problem, we use the combined gas law which is given below:

        [tex]\frac{P1 V1}{T1}[/tex]    = [tex]\frac{P2 V2}{T2}[/tex]  

P, V and T are pressure, volume and temperature

1 and 2 are initial and new states

                  [tex]\frac{8.5 x 75}{288}[/tex]   = [tex]\frac{1.7 x 6.1}{T2}[/tex]

        T2  = 4.7K

What is the measure of the amount of dissolved salts in ocean water?

Answers

Answer:

Salinity

HOPE THIS HELPS! :)

Explanation:

use scientific notation to solve

Answers

5.8x1015
Hope this helped :)

which of the following is a strong acid?
A, HCI (hydrochloric acid)
B, HF (hydrofluoric acid)
C, NaOH (sodium hydroxide)
D, NH3 (ammonia)

Answers

HCI is the strongest out of those.

Twelve grams of gas occupies 14.5 liters at a pressure of 52.0cm Hg. What is the volume when the pressure has increased to 85.0cm Hg?

Answers

Answer:

8.87 L.

Explanation:

From the question given above, the following data were obtained:

Mass (m) of gas = 12 g

Initial volume (V₁) = 14.5 L

Initial pressure (P₁) = 52 cmHg

Final pressure (P₂) = 85 cmHg

Final volume (V₂) =?

The final volume of the gas can be obtained by using the Boyle's Las equation as illustrated below:

P₁V₁ = P₂V₂

52 × 14.5 = 85 × V₂

754 = 85 × V₂

Divide both side by 85

V₂ = 754 / 85

V₂ = 8.87 L

Thus the volume of the gas will be 8.87 L

What did organizing information on the periodic table reveal?
A. Subatomic particles
B. New forms of energy
C. Molecular structures
D. Periodic patterns and trends

Answers

Answer:

The groupings of elements in the periodic table reveal various trends, present in groups such as the alkali metals and noble gases, or the lanthanides and actinides. The periodic table can be used to find information on elements or predict the properties of as-of-yet undiscovered elements.

Explanation:

Answer: D

Just took the quiz

US
What is the percent of H in
NH3?
(H = 1.008 amu, N = 14.01 amu)
[?]%

Answers

Answer:

17.6

Explanation:

Answer is explained above

3/17*100=17.6
It would be 17.6%.

1. What are the two effects of the continental plate/continental plate convergent boundary?

2. Where can you find an example of this type of divergent boundary? Which country, continent or ocean?

Answers

Answer

oh you again?

Explanation:

___________ changes affect the nuclei (nucleus) of an atom, which produces new elements. (A Nuclear (B Chemical (C Physical

Answers

Answer:

A

Explanation:

Is Gold (Au) soluble?

Answers

Answer:

yes

Explanation:

A solution was diluted 1/40, and the final concentration was 20%. What was the original concentration?

Answers

The original concentration : 800%

Further explanation

Given

Diluted 1/40

Final concentration 20%

Required

The original concentration

Solution

Dilution is the process of adding solvent to get a more dilute solution.

The moles(n) before and after dilution are the same.

Can be formulated :

 n₁ = n₂

M₁.V₁ = M₂.V₂

diluted 1/40(dilution factor)⇒ V₁/V₂=1/40⇒V₂/V₁=40

M₂ = 0.2(20%)

Input the value for M₁ :

M₁=M₂ x (V₂/V₁)

M₁ = 0.2 x 40

M₂ = 8(800%)

=

What is Sr(NO3)2 + ZN ?

Answers

Answer:

strontium nitrate

Explanation:

hope this helps you....:)

What are important things we can figure out by looking at a food web?

Answers

Answer:

We can find out what animals eat..and how long a food chain could get..?

Explanation:

Energy transfer between organisms

Measure that affects the thermal energy of matter but not it's temperature. ​

Answers

Answer: mass

Explanation:

Mr. Smith wanted to see if the color of light shined on a plant had an effect on the number of leaves it had. He gathered a 2
groups of the same species of plants, gave them the same amount of water, and did the test for the same amount of time.
On one group of plants he used white light. On the second group he changed the light color to red. Identify:
Independent variable:
Dependent variable:
Constants:
Control group (What would Mr. Smith use as a control group?):
Experimental group (what would Mr. Smith use as a experimental group?)

Answers

Higgffggvtgrjtbrb verve even

The table below shows properties of two compounds at standard pressure. Which statement classifies the two compounds? 1)Both compounds are ionic, 2)Both compounds are molecular, 3) Compound 1 is ionic, and compound 2 is molecular, 4)Compound 1 is molecular, and compound is ionic

Answers

Answer:

3) Compound 1 is ionic, and compound 2 is molecular

Explanation:

Compound-1 is an ionic compound while Compound-2 is a molecular compound. Option 3 is correct.

Ionic Compound:

They are formed when an atom donates its electron to another atom. This results in one positive and one negative ion.

Hence, it is a very strong bond. These compounds have a very high melting and boiling point. They are very good electric conductors in the aqueous solution.

For example- NaCl.

Molecular compounds:

They are compounds that are made up of discrete molecular units. For example- [tex]\bold {H_2O}[/tex]

They have very weak intermolecular bonds hence have low boiling and melting point and do not conduct electricity.

SInce Compound-1 has higher MP and BP and Compound-2 has low melting and boiling point.

Therefore, Compound-1 is an ionic compound while Compound-2 is a molecular compound.

To know more about Ionic Compound:

https://brainly.com/question/9167977

How many moles are in 1.23 x 1024 molecules of oxygen gas.

Answers

Answer:

2.04moles

Explanation:

Given parameters:

Number of molecules  = 1.23 x 10²⁴molecules

Unknown:

Number of moles of oxygen gas = ?

Solution:

To solve this problem;

                  6.02 x 10²³ molecules makes up 1 mole of any substance

                1.23 x 10²⁴molecules will make up [tex]\frac{1.23 x 10^{24} }{6.02 x 10^{23} }[/tex]   = 2.04moles

6.02 x 10²³ molecules makes up 1 mole of any substance

               1.23 x 10²⁴molecules will make up = 2.04moles

(9) How many moles of oleum in 1780. g of oleum? - Write an equation to summarize the process.

Answers

Answer:

1. 10moles

2. Find the summarized equation below

Explanation:

Mole = mass ÷ molar mass

Molar mass of oleum (H2S2O7) = 1(2) + 32(2) + 16(7)

= 2 + 64 + 112

= 178g/mol

According to the information provided, the mass of oleum is 1780g, hence, the number of moles in it is:

Mole = 1780/178

Mole = 10moles

2. The summarized equation of the contact process is:

1) S + O2 → SO2

2) 2SO2 + O2 → 2SO3

3) SO3 + H2SO4 (conc. sulfuric acid) → H2S2O7

4) H2S2O7 + H2O → 2H2SO4 (dilute sulfuric acid)

As a heavy metal ball rolls down a hill, it goes faster and faster. Which statement is true?

Answers

The ball's potential energy is transferring into kinetic energy

Brainly if correct? Thanks!

Answer:

A

Explanation:

The balls potential energy is changing to kinetic energy

All moving objects have kinetic energy. True False

Answers

Answer:

true

.......................

Answer: You're Welcome!

Oh, It's False - Kinetic energy depends upon the speed of the object; potential energy depends upon the position of the object.

Electromagnetic waves travel because of ____

Answers

because they need a medium to travel they can travel everywhere if they got the medium

Propane is what type of matter?

Element
Compound
Homogeneous mixture
Heterogeneous mixture

Answers

Propane is a compound of Carbon and Hydrogen
CH4 is a compound because it is Made of carbon and H

How do the percent compositions for C3H6 and C4H7 compare?
A. They are the same
B. C4H8 has a higher percentage of carbon than C3H6.
C. C4H8 has a higher percentage of hydrogen than C3H6.
D. none of the above

Answers

A. They are the same

Further explanation

Given

C3H6 and C4H8

Required

The percent compositions

Solution

C₃H₆(MW = 42 g/mol)

%C = 3.12/42 x 100% = 85.71%

%H = 6.1/42 x 1005 = 14.29%

C₄H₈(MW=56 g/mol)

%C = 4.12/56 x 100% = 85.71%

%H = 8.1/56 x 100%=14.29%

So they are the same, because mol ratio of C and H in both compounds is the same, 1: 2

A chemist adds 600.0mL of a 0.444/gL calcium sulfate CaSO4 solution to a flask. Calculate the mass in grams of calcium sulfate the chemist has added to the flask. Be sure your answer has the correct number of significant digits.

Answers

Answer:

0.266 grams of calcium sulfate

Explanation:

We can calculate the mass of calcium sulfate added using the given volume and concentration of the solution:

First we convert 600.0 mL to L ⇒ 600.0 mL equals 0.6000 L (600 ÷ 1000).

0.444 g/L * 0.6000 L = 0.266 g

Thus the answer is 0.266 grams of calcium sulfate.

How many grams of water are produced when 37.7 g of hydrogen peroxide (H2O2) decomposes into oxygen gas and water?

Answers

Answer:

Mass of water produced = 19.8 g

Explanation:

Given data:

Mass of hydrogen peroxide = 37.7 g

Mass of water produced = ?

Solution:

Chemical equation:

2H₂O₂      →        2H₂O  + O₂

Number of moles of H₂O₂:

Number of moles = mass/molar mass

Number of moles = 37.7 g/ 34 g/mol

Number of moles = 1.1 mol

Now we will compare the moles of H₂O₂ and H₂O from balance chemical equation in order to determine the moles of water formed.

                                   H₂O₂      ;           H₂O

                                      2         :             2

                                     1.1         :          1.1

Mass of water formed:

Mass = number of moles × molar mass

Mass = 1.1 mol × 18 g/mol

Mass = 19.8 g

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What words, phrases,or ideas do you findmost meaningful in thespeech?I had a dream Which of the following is true about the Battle of New Orleans? A. This was the first battle of the War of 1812B. It was unnecessary because the peace treaty had already been signed C. Andrew Jackson led the British to victoryD. It was a decisive victory for the British What is the value of (4)5/(4)3? A third figure of speech Twain uses to good effect is personification. When you represent a thing, quality, or idea as if it were a human person, you are using personification. Familiar examples are Mother Nature, Jack Frost, and Uncle Sam. This story contains good examples of personification in the description of Andrew Jackson, the dog, and Dan'l Webster, the frog. Both are given some human qualities.List one example from the descriptions of these animals that shows them to be human, at least in Simon Wheeler's opinion. The data below shows the change in concentration of dinitrogen pentoxide over time, at 330 K, according to the following process.2N2O5(g) = 4NO2(g) + O2[N2O5] Time (s)0.100 0.000.066 200.000.044 400.00a) Find the rate of disappearance of N2O5 from t=0 s to t=200sb) Find the rate of appearance of NO2 from t=0 s to t =200s Which of the following sentences correctly uses quotation marks?A.The "summer" is going to be nice, she said.B."The summer is going to be nice", she said.C."The summer is going to be nice," she said.D.The summer is going to be nice, "she said." Consider social stratification from the symbolic interactionist perspective. How does social stratification influence the daily interactions of individuals? Illustrate your ideas with specific examples and anecdotes from your own life and the lives of people in your community.. Single line text. please help me my brain is to small for dis I need da right answer In the case of an expansionary_____policy, the interest rate rises, while in the case of an expansionary _____ policy, the interest rate falls.Select one:a. fiscal; monetaryb. monetary, monetaryc. monetary, fiscald. fiscal; fiscal= fiscal; monetary hola i need sum help pls Now that you know more about why World War I broke out and how the terms of peace were intended to prevent it from happening again, do you believe that the leaders who drafted the Treaty of Versailles did a good job? I can do your homework for you 15Is this rational or irrational Help brainliest True or FalseIn Python, the first character in a string has an index position of one.TRUE/False 1. What is the slope of a line that is perpendicular to the x-axis Combine the like terms to create an equivalent expression:y-(-3y)I think this is the last oneLOL....... Cul es un platillo tpico de Espaa?A.gallo pintob. paellac. frijoles Where is the person in the poem invierno tardio, what does he look at? Help plssss with my exit ticket