Answer:
ur mom???
Explanation:
PLEASEEE HELP!! Ill mark brainlist
How/why does the DNA separate?
Discuss porosity or pores, electricity, DNAS charge & size of DNA pieces. Highlight all the 4 terms
Electrophoresis is a technique commonly used in the lab to separate charged molecules, like DNA, according to size.
Gel electrophoresis is a technique commonly used in laboratories to separate charged molecules like DNA?, RNA? and proteins? according to their size.
Charged molecules move through a gel when an electric current is passed across it.
An electric current is applied across the gel so that one end of the gel has a positive charge and the other end has a negative charge.
The movement of charged molecules is called migration. Molecules migrate towards the opposite charge. A molecule with a negative charge will therefore be pulled towards the positive end (opposites attract!).
The gel consists of a permeable matrix, a bit like a sieve, through which molecules can travel when an electric current is passed across it.
Smaller molecules migrate through the gel more quickly and therefore travel further than larger fragments that migrate more slowly and therefore will travel a shorter distance. As a result the molecules are separated by size.
Gel electrophoresis and DNA
Electrophoresis enables you to distinguish DNA fragments of different lengths.
DNA is negatively charged, therefore, when an electric current is applied to the gel, DNA will migrate towards the positively charged electrode.
Shorter strands of DNA move more quickly through the gel than longer strands resulting in the fragments being arranged in order of size.
The use of dyes, fluorescent? tags or radioactive? labels enables the DNA on the gel to be seen after they have been separated. They will appear as bands on the gel.
A DNA marker with fragments of known lengths is usually run through the gel at the same time as the samples.
By comparing the bands of the DNA samples with those from the DNA marker, you can work out the approximate length of the DNA fragments in the samples.
How is gel electrophoresis carried out?
Preparing the gel
Agarose gels? are typically used to visualise fragments of DNA. The concentration of agarose used to make the gel depends on the size of the DNA fragments you are working with.
The higher the agarose concentration, the denser the matrix and vice versa. Smaller fragments of DNA are separated on higher concentrations of agarose whilst larger molecules require a lower concentration of agarose.
To make a gel, agarose powder is mixed with an electrophoresis buffer and heated to a high temperature until all of the agarose powder has melted.
The molten gel is then poured into a gel casting tray and a “comb” is placed at one end to make wells for the sample to be pipetted into.
Once the gel has cooled and solidified (it will now be opaque rather than clear) the comb is removed.
Many people now use pre-made gels.
The gel is then placed into an electrophoresis tank and electrophoresis buffer is poured into the tank until the surface of the gel is covered. The buffer conducts the electric current. The type of buffer used depends on the approximate size of the DNA fragments in the sample.
Preparing the DNA for electrophoresis
A dye is added to the sample of DNA prior to electrophoresis to increase the viscosity of the sample which will prevent it from floating out of the wells and so that the migration of the sample through the gel can be seen.
A DNA marker (also known as a size standard or a DNA ladder) is loaded into the first well of the gel. The fragments in the marker are of a known length so can be used to help approximate the size of the fragments in the samples.
The prepared DNA samples are then pipetted into the remaining wells of the gel.
When this is done the lid is placed on the electrophoresis tank making sure that the orientation of the gel and positive and negative electrodes is correct (we want the DNA to migrate across the gel to the positive end).
Separating the fragments
The electrical current is then turned on so that the negatively charged DNA moves through the gel towards the positive side of the gel.
Shorter lengths of DNA move faster than longer lengths so move further in the time the current is run.
The distance the DNA has migrated in the gel can be judged visually by monitoring the migration of the loading buffer dye.
The electrical current is left on long enough to ensure that the DNA fragments move far enough across the gel to separate them, but not so long that they run off the end of the gel.
Illustration of DNA electrophoresis equipment used to separate DNA fragments by size. A gel sits within a tank of buffer. The DNA samples are placed in wells at one end of the gel and an electrical current passed across the gel. The negatively-charged DNA moves towards the postive electrode. Image credit: Genome Research Limited
tank.
are environmental factors more likely to affect genotype or phenotype?
Environmental factors are more likely to affect phenotype than genotype.
Phenotype refers to the observable physical characteristics of an organism, a product of the genetic information contained in the genotype, in accordance with the conditions of the determined environment in which the organism lives.
The phenotype supposes the manifestation of the genotype based on the environmental factors, the traits expressed by the phenotype are behavioral and physical.Regardless of the genotypic information manifested, environmental factors will also shape the phenotype of an organism, since it can suffer accidents, changes or adaptations that a genetically similar individual but in another environment would not suffer.Therefore, we can conclude that the phenotype is the variable expression of a genotype in accordance with the environmental factors.
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A student is researching the inheritance patterns of different traits in chickens. She hypothesizes that red feathers are dominant over black, and the trait for crests are dominant over non-crested. She performs a test cross to confirm her hypothesis. To produce the F1 generation, she crosses two parents-- a chicken that is homologous for traits showing red and crested and a chicken that is homologous for traits showing black and non-crested. Which ratio best represents the phenotype of the F2 generation
9:3:3:1
1:1:1:1
2:2:1:1
16:0
Answer:
9:3:3:1
Explanation:
In this inheritance pattern, the ratio that best represents the phenotype of the F2 generation is 9:3:3:1. Thus, the correct option for this question is A.
What is a Phenotype?A phenotype may be defined as the mechanism of expressing genotypes in the form of traits. In more simple words, it is also characterized as the morphology or physiology which comes due to the combination of alleles.
If a trait of red feathers is denoted by R and the trait of black feathers is denoted by r, then R > r. Similarly, a trait of crests is denoted by C and the trait of non-crested is denoted by c, then, C > c.
Now, RRCC is crossed with rrcc, the F1 offspring have the genotype RrCc with the phenotype of red feathers with crests. Now, RrCc is selfed, it produces the ratio of 9:3:3:1. This is an example of a dihybrid cross.
Therefore, in this inheritance pattern, the ratio that best represents the phenotype of the F2 generation is 9:3:3:1. Thus, the correct option for this question is A.
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As a result of an injury in gallbladder of a person, it had been removed surgically, which of the following processes can be affected by this? *
a. The removal of amino groups of proteins.
B. The digestion of carbohydrates.
C. The breaking down of peptide bonds of proteins.
D. The digestion of fats
Answer:
D
Explanation:
Ribosomes are the site where— are produced.
Amino acids are coded for by triplet bases in RNA called—.
Answer:
Ribosomes are the site where proteins are produced. Amino acids are coded for by triplet bases in RNA called codons
Explanation:
the step in kidney function in which fluid is forced out of the blood includes moving fluid __________.
Answer:
The step in kidney function which fluid is forced out of the blood is from glomerulus to glomerular capsule.
Explanation:
help meeeeeeeeeeeeeeeeeeeeeeeee
Answer:
Question is not presented
Explanation:
1. How has the Exxon Valdez oil spill
impacted the environment?
Answer:
The oil killed and injured seabirds, sea otters, harbor seals, bald eagles, orcas and other wildlife. Eventually, oil from the spill affected more than 1,000 miles of Alaska's remote and rugged coastline.
Other Info That Might Help:
Image result for How has the Exxon Valdez oil spill impacted the environment? Environmental And Economic Impacts Prince William Sound had been a pristine wilderness before the spill. The Exxon Valdez disaster dramatically changed all of that, taking a major toll on wildlife. It killed an estimated 250,000 sea birds, 3,000 otters, 300 seals, 250 bald eagles and 22 killer whales.
Explanation:
I have answered This before and I got it right.
Hope this helps! :)
How do rocks become igneous rock?
Answer:
Igneous rocks form when magma (molten rock) cools and crystallizes
Explanation:
So first when the sedimentary rock is formed and adds some heat pressure in which it turns to metamorphic rock, and after a long time heat + cool down which it will turn to igneous rock!
Protists are organized into the following six groups: Excavata, Chromalveolata, Rhizaria, Archaeplastida, Amoebozoa, and Ophisthokonta. What are these groups called
Answer:Major Groups of Protists
Explanation:
What substances in food provide organisms with energy?
Answer:
A food is something that provides nutrients. Nutrients are substances that provide: energy for activity, growth, and all functions of the body such as breathing, digesting food, and keeping warm; materials for the growth and repair of the body, and for keeping the immune system healthy.
Explanation:
Answer: Carbohydrates
Explanation: Because of the starches and sugars in food, that gives your body‘s main sources of energy.
The concept of evolution by way of natural selection is a central tenet in biology that has been tested in numerous ways by many scientists. Evolution by way of natural selection is most likely which type of concept?
A. an explanation of phenomena that can be accepted as true because it is supported by repeated observations and experimentation
B. a likelihood based on the chance that a particular event will or will not occur during an experiment
C. a testable explanation or answer to a scientific question based on prior knowledge or research
D. a statement of fact that is accepted as true because it has always been observed to be true through observations and experimentation
Answer:
I know I am too late to help you, but I couldn't find an answer when looking for this question. I got it right and it is A. an explanation of phenomena that can be accepted as true because it is supported by repeated observations and experimentation.
Explanation:
Evolution by way of natural selection is most likely an explanation of phenomena that can be accepted as true because it is supported by repeated observations and experimentation. The correct option is A.
What is natural selection?Natural selection is the process through which organisms that are more adapted to their environment tend to survive and reproduce more than those that are not. Tree frogs, for example, are occasionally eaten by snakes and birds.
Evolution is a process in which living things or other evolve or change over time. Natural selection is also a reason for evolution, which takes a large amount of time.
Therefore, the correct option is A. an explanation of phenomena that can be accepted as true because it is supported by repeated observations and experimentation
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How to find recombination frequency.
Answer:
A test cross is required
Which two atoms form an ionic bond?
A.sodium and bromine
B. two sodium atoms
C. two oxygen atoms
D fluorine and chlorine
Answer:
sodium and bromine
Explanation:
The one valence electron from sodium would be transferred to the bromine atom so they can both have an octet.
Which two atoms form an ionic bond?
Answer : A
Explanation : Ionic bond is a type of chemical bond and binds the chemical molecules together. Ionic bond is formed by the transfer of electrons from an electropositive element ( metal) to the electronegative element (non metal).
Sodium is an electropositive element and contains one positive charge. Bromine is an electronegative element with a single negative charge over it. Sodium gives the electron to bromine and results in the formation of ionic bond.
Thus, the correct answer is option (C).
Hope this helps you!
a section of a dna molecule that controls the thumb is called a
Answer:
gene
Explanation:
Which part of the vascular system functions as a blood reservoir and contains over 60% of the body's blood
Answer:
Veins are also called capacitance vessels because they contain 60% of the body's blood volume.
Explanation:
Hope I could help.
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! What landforms could be the result of diverging plates and name a location where this occurs?
Answer:
rift valleys and mid-oceanic ridges.
Explanation:
the largest and most numerous of the glial cells in the central nervous system are the
Answer:
The largest and most numerous of the glial cells in the central nervous system are the astrocytes
Explanation:
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In the citric acid cycle, the enzyme succinate dehydrogenase catalyzes a reversible reaction using FAD/FADH2 as an oxidizing/reducing agent. Which of the following statements best explains how the redox component of this reaction contributes to the reaction's ability to be reversible under cellular conditions?
A. FAD has no reduction potential when it is enzyme-bound.
B. FAD can be reduced by the sequential addition of one hydrogen at a time.
C. FAD/FADH2 is covalently bound to succinate dehydrogenase.
D. The change in the biochemical standard reduction potential is small.
The statement which best explains how the redox component of this reaction contributes to the reaction's ability to be reversible under cellular conditions is; Choice D: The change in the biochemical standard reduction potential is small.
Discussion:
A reversible process is one in which the system and environment can be restored to exactly the same initial states that they were in prior to when the process occurred, if we go backward along the path of the process.
However, the necessary condition for a reversible process is therefore the quasi-static requirement.The quasi-static requirement in this case is that the change in the biochemical standard reduction potential is small.Read more:
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Black hair (b) is dominant over brown hair (b) in guinea pigs. What is the genotype of a brown guinea pig?.
Answer:
the genotype of the brown guinea pig would be 'bb'
Explanation:
if it had black hair the possible genotypes would be 'Bb' or 'BB' because any genotype with a capital letter is dominant and brown hair is a recessive trait I'm assuming considering the question says "over"
Biology cell division
What are the answers to this chart
The cell spends most of the time in the interphase, which is in the G1, S, and G2 phases, and approximately 22 hours out of 24 hours, with the rest being 1–2 hours, are spent in the division phase.
What is the time spent in the cell cycle?
The cell enters the cell cycle, and the first stage is the G1 phase, where the cell grows and spends a lot of time nearly 11 hours and then the cell enters the S phase. In the S phase, the DNA replicates and spends nearly 7 hours, and then in the G2 phase, 3 to 4 hours are spent by the cell, with the remaining 1 to 2 hours spent in the M phase, also called the mitosis or division phase.
Hence, cells spend most of the time in the interphase, which is in the G1, S, and G2 phases, and approximately 22 hours out of 24 hours, with the rest being 1–2 hours, are spent in the division phase.
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Which of the following snack foods is comprised mainly of carbohydrates?
Answer:
d. All of the above
Explanation:
Is it True or false?
Plzzzzz help meeee plzzzz
Answer:
The answer is true..
Yes, the presticides cause aquatic animals like fishes to die..
Mitosis is a type of cell division. organize the images below to show the steps of mitosis.
(QUICK PLZ)
Answer:
i believe its the first
Explanation:
people are not allow to enter the Wild reserves why
Answer:
because it can be very dangerous depending on the animal
Explanation:
hope this helps
Answer:
Because in wild life reserve, endangered animals are protected. So, people are not allowed to enter the Wild reservers
4. Use the atomic model below to answer the following questions: 1 pt for each blank
How many protons?
How many neutrons?
What is the name of this atom?
Answer:
for first 1 its 3 nuetros i think
state the expressed power you think is the most important and explain why you think it is the most important.
Answer:
The most important powers include the power to tax, to borrow money, to regulate commerce and currency, to declare war, and to raise armies and maintain the navy. These powers give Congress the authority to set policy on the most basic matters of war and peace.
Explanation: If I am wrong then i am sorry
Sperm cells have a whip like ____ to move them through a liquid.
Answer:
tail
Explanation:
Answer:
flagellum
Explanation:
a slender threadlike structure, especially a microscopic appendage that enables many protozoa, bacteria, spermatozoa.
You touch a hot pan when cooking. List out the steps, in detail, of the nervous pathway of the reflex that occurs. Include any sensory organs involved and the action of the reflex.